3D image datasets are at the heart of webKnossos. Import datasets via the file system or the upload feature. Configure the dataset defaults and permissions to your specification. Share your datasets with the public or with selected users.
If your dataset is not yet in WKW or KNOSSOS format, you need to convert it. The webKnossos Cuber is a tool that can convert many formats to WKW in order to be used with webKnossos. Read more in the Data Formats documentation.
To efficienty import large datasets, we recommend to place them directly in the file system:
Place the dataset at
<webKnossos directory>/binaryData/<Organization name>/<Dataset name>. For example
Go to the dataset view on the dashboard
Use the refresh button on the dashboard or wait for webKnossos to detect the dataset (up to 10min)
Import for your new dataset
Complete the Import screen
To quickly import a dataset, you may use the upload functionality from webKnossos. This is only recommended for datasets up to 1 GB.
In order to upload the datasets, create a ZIP file that contains the WKW or KNOSSOS cubes in the folder structure as described in the Data Formats guide. Once the data is uploaded you need to complete the Import screen.
The Import screen allows you to set some properties of your datasets. Many properties such as available layers, bounding boxes and datatypes can be detected automatically. Some properties require your manual input, though. Most of the time these are scale which represents the physical size of one voxel in nanometers and largestSegmentId of a segmentation layer.
Once you entered the required properties, you can click the
Import button to complete the process. The dataset is now ready to use.
A list of sample datasets is provided with webKnossos. Click
Add a Sample Dataset on the upload page to access it and choose datasets to be added and imported automatically. The three sample datasets currently available are:
Sample_e2006_wkw: https://static.webknossos.org/data/e2006_wkw.zip Raw SBEM data and segmentation (sample cutout, 120MB). Connectomic reconstruction of the inner plexiform layer in the mouse retina. M Helmstaedter, KL Briggman, S Turaga, V Jain, HS Seung, W Denk. Nature. 08 August 2013. https://doi.org/10.1038/nature12346
Sample_FD0144_wkw: https://static.webknossos.org/data/FD0144_wkw.zip Raw SBEM data and segmentation (sample cutout, 316 MB). FluoEM, virtual labeling of axons in three-dimensional electron microscopy data for long-range connectomics. F Drawitsch, A Karimi, KM Boergens, M Helmstaedter. eLife. 14 August 2018. https://doi.org/10.7554/eLife.38976
Sample_MPRAGE_250um: https://static.webknossos.org/data/MPRAGE_250um.zip MRI data (250 MB). T1-weighted in vivo human whole brain MRI dataset with an ultrahigh isotropic resolution of 250 μm. F Lüsebrink, A Sciarra, H Mattern, R Yakupov, O Speck. Scientific Data. 14 March 2017. https://doi.org/10.1038/sdata.2017.32
You can edit the properties of a dataset at any time. In addition to the required properties that you need to fill in during import, there are more advanced properties that you can set. This screen is similar to the Import screen and split into three tabs:
Scale: The physical size of a voxel in nanometers, e.g.
11, 11, 24
Bounding Box: The position and extents of the dataset layer in voxel coordinates. The format is
x,y,z,x_size,y_size,z_size or respectively
Largest Segment ID: The highest ID that is currently used in the respective segmentation layer. This is required for volume annotations where new objects with incrementing IDs are created. Only applies to segmentation layers.
Advanced view lets you edit the underlying JSON configuration directly.
Display Name: Used as the name of the dataset in the Gallery view.
Description: Contains more information about your datasets including authors, paper reference, descriptions. Supports Markdown formatting. The description will be featured in the Gallery view as well.
Allowed Teams: Defines which teams of your organization have access to this dataset. By default no team has access but admins and team managers can see and edit the dataset.
Visibility: Lets you make the dataset available to the general public and shows it in the public Gallery view. This will enable any visitor to your webKnossos instance to view the data, even unregistered users.
Sharing Link: A special URL which allows any user to view your dataset that uses this link. Because of the included random token, the link cannot be guessed by random visitors. You may also revoke the random token and create a new one when you don't want previous users to access your data anymore. Read more in the Sharing guide.
Position: Default position of the dataset in voxel coordinates. When opening the dataset, users will be located at this position.
Zoom: Default zoom.
Segmentation Opacity: Default opacity of the segmentation layer.
Interpolation: Whether interpolation should be enabled by default.
Layer Configuration: This is an advanced feature to control the default settings (e.g. brightness, contrast, color) per layer. It needs to be configured in a JSON format.
Read more in the Sharing guide
The system architecture of webKnossos allows for versatile deployment options where you can install a dedicated datastore server directly on your lab's cluster infrastructure. This may be useful when dealing with large datasets that should remain in your data center. Please contact us if you require any assistance with your setup.
We also offer dataset alignment services, click here to find out more.